Construct: shRNA TRCN0000035439
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_015039.2-894s1c1
- DNA Barcode:
- CGGTATGAAGAGATTGAGCTA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NMNAT2 (23057)
- Gene Description:
- nicotinamide nucleotide adenylyltransferase 2
- Transcript:
- RefSeq NM_015039.2 (NON-CURRENT)
- Match location:
- Position 894 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CGGTATGAAGAGATTGAGCTA
- Hairpin Sequence:
- 5'-CCGG-CGGTATGAAGAGATTGAGCTA-CTCGAG-TAGCTCAATCTCTTCATACCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGTATGAAGAGATTGAGCTACTCGAGTAGCTCAATCTCTTCATACCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGTATGAAGAGATTGAGCTACTCGAGTAGCTCAATCTCTTCATACCG-3'