Construct: shRNA TRCN0000035448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005787.3-612s1c1
DNA Barcode:
CCTGGCAGTCTCTGTGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALG3 (10195)
Gene Description:
ALG3 alpha-1,3- mannosyltransferase
Transcript:
RefSeq NM_005787.3 (NON-CURRENT)
Match location:
Position 612 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCAGTCTCTGTGAAGAT
Hairpin Sequence:
5'-CCGG-CCTGGCAGTCTCTGTGAAGAT-CTCGAG-ATCTTCACAGAGACTGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCAGTCTCTGTGAAGATCTCGAGATCTTCACAGAGACTGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCAGTCTCTGTGAAGATCTCGAGATCTTCACAGAGACTGCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000288749