Construct: shRNA TRCN0000035458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004482.2-313s1c1
DNA Barcode:
GCAGAATTGAAGCCTGTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALNT3 (2591)
Gene Description:
polypeptide N-acetylgalactosaminyltransferase 3
Transcript:
RefSeq NM_004482.2 (NON-CURRENT)
Match location:
Position 313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATTGAAGCCTGTCCTT
Hairpin Sequence:
5'-CCGG-GCAGAATTGAAGCCTGTCCTT-CTCGAG-AAGGACAGGCTTCAATTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATTGAAGCCTGTCCTTCTCGAGAAGGACAGGCTTCAATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATTGAAGCCTGTCCTTCTCGAGAAGGACAGGCTTCAATTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000288829