Construct: shRNA TRCN0000035480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024105.2-485s1c1
DNA Barcode:
CTGTTAGAAATGTCCAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALG12 (79087)
Gene Description:
ALG12 alpha-1,6-mannosyltransferase
Transcript:
RefSeq NM_024105.2 (NON-CURRENT)
Match location:
Position 485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTAGAAATGTCCAAGTTT
Hairpin Sequence:
5'-CCGG-CTGTTAGAAATGTCCAAGTTT-CTCGAG-AAACTTGGACATTTCTAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTAGAAATGTCCAAGTTTCTCGAGAAACTTGGACATTTCTAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTAGAAATGTCCAAGTTTCTCGAGAAACTTGGACATTTCTAACAG-3'

Other clones with same target sequence:

(none)