Construct: shRNA TRCN0000035500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006100.2-1332s1c1
DNA Barcode:
CCTTTGCACTACTATGGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST3GAL6 (10402)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Transcript:
RefSeq NM_006100.2 (NON-CURRENT)
Match location:
Position 1332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGCACTACTATGGGAAT
Hairpin Sequence:
5'-CCGG-CCTTTGCACTACTATGGGAAT-CTCGAG-ATTCCCATAGTAGTGCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGCACTACTATGGGAATCTCGAGATTCCCATAGTAGTGCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGCACTACTATGGGAATCTCGAGATTCCCATAGTAGTGCAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000288832