Construct: shRNA TRCN0000035551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003033.2-1602s1c1
DNA Barcode:
GCTGGGAGATAATGTCAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST3GAL1 (6482)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 1
Transcript:
RefSeq NM_003033.2 (NON-CURRENT)
Match location:
Position 1602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGAGATAATGTCAGCAT
Hairpin Sequence:
5'-CCGG-GCTGGGAGATAATGTCAGCAT-CTCGAG-ATGCTGACATTATCTCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGAGATAATGTCAGCATCTCGAGATGCTGACATTATCTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGAGATAATGTCAGCATCTCGAGATGCTGACATTATCTCCCAGC-3'

Other clones with same target sequence:

(none)