Construct: shRNA TRCN0000035554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145292.2-1136s1c1
DNA Barcode:
CCTGCAATGTCTGGAGGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALNTL5 (168391)
Gene Description:
polypeptide N-acetylgalactosaminyltransferase like 5
Transcript:
RefSeq NM_145292.2 (NON-CURRENT)
Match location:
Position 1136 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAATGTCTGGAGGAATT
Hairpin Sequence:
5'-CCGG-CCTGCAATGTCTGGAGGAATT-CTCGAG-AATTCCTCCAGACATTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAATGTCTGGAGGAATTCTCGAGAATTCCTCCAGACATTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAATGTCTGGAGGAATTCTCGAGAATTCCTCCAGACATTGCAGG-3'

Other clones with same target sequence:

(none)