Construct: shRNA TRCN0000035606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020474.2-461s1c1
DNA Barcode:
CCAAGACACATGATAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALNT1 (2589)
Gene Description:
polypeptide N-acetylgalactosaminyltransferase 1
Transcript:
RefSeq NM_020474.2 (NON-CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGACACATGATAGAAGAA
Hairpin Sequence:
5'-CCGG-CCAAGACACATGATAGAAGAA-CTCGAG-TTCTTCTATCATGTGTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGACACATGATAGAAGAACTCGAGTTCTTCTATCATGTGTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGACACATGATAGAAGAACTCGAGTTCTTCTATCATGTGTCTTGG-3'

Other clones with same target sequence:

(none)