Construct: shRNA TRCN0000035762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003596.2-1366s1c1
DNA Barcode:
CGAAGGGTCTATAAGGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPST1 (8460)
Gene Description:
tyrosylprotein sulfotransferase 1
Transcript:
RefSeq NM_003596.2 (NON-CURRENT)
Match location:
Position 1366 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGGGTCTATAAGGGAGAA
Hairpin Sequence:
5'-CCGG-CGAAGGGTCTATAAGGGAGAA-CTCGAG-TTCTCCCTTATAGACCCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGGGTCTATAAGGGAGAACTCGAGTTCTCCCTTATAGACCCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGGGTCTATAAGGGAGAACTCGAGTTCTCCCTTATAGACCCTTCG-3'

Other clones with same target sequence:

(none)