Construct: shRNA TRCN0000035822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_114301.5-413s1c1
DNA Barcode:
CCTTCTCTTGTCTGTGTGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALG1L (200810)
Gene Description:
ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase like
Transcript:
RefSeq XM_114301.5 (NON-CURRENT)
Match location:
Position 413 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTCTTGTCTGTGTGATA
Hairpin Sequence:
5'-CCGG-CCTTCTCTTGTCTGTGTGATA-CTCGAG-TATCACACAGACAAGAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTCTTGTCTGTGTGATACTCGAGTATCACACAGACAAGAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTCTTGTCTGTGTGATACTCGAGTATCACACAGACAAGAGAAGG-3'

Other clones with same target sequence:

(none)