Construct: shRNA TRCN0000035841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001263.2-850s1c1
DNA Barcode:
GCTTCCATGAAATTATCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDS1 (1040)
Gene Description:
CDP-diacylglycerol synthase 1
Transcript:
RefSeq NM_001263.2 (NON-CURRENT)
Match location:
Position 850 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCATGAAATTATCACTA
Hairpin Sequence:
5'-CCGG-GCTTCCATGAAATTATCACTA-CTCGAG-TAGTGATAATTTCATGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCATGAAATTATCACTACTCGAGTAGTGATAATTTCATGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCATGAAATTATCACTACTCGAGTAGTGATAATTTCATGGAAGC-3'

Other clones with same target sequence:

(none)