Construct: shRNA TRCN0000035982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376573.1-79s1c1
DNA Barcode:
GCATCTTTCTTTAAAGGGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC401305 (401305)
Gene Description:
similar to beta-1,4-mannosyltransferase
Transcript:
RefSeq XM_376573.1 (NON-CURRENT)
Match location:
Position 79 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTTTCTTTAAAGGGACA
Hairpin Sequence:
5'-CCGG-GCATCTTTCTTTAAAGGGACA-CTCGAG-TGTCCCTTTAAAGAAAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTTTCTTTAAAGGGACACTCGAGTGTCCCTTTAAAGAAAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTTTCTTTAAAGGGACACTCGAGTGTCCCTTTAAAGAAAGATGC-3'

Other clones with same target sequence:

(none)