Construct: shRNA TRCN0000036038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000436.1-1312s1c1
DNA Barcode:
CCAAATATGGTGACCTGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OXCT1 (5019)
Gene Description:
3-oxoacid CoA-transferase 1
Transcript:
RefSeq NM_000436.1 (NON-CURRENT)
Match location:
Position 1312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATATGGTGACCTGGCTA
Hairpin Sequence:
5'-CCGG-CCAAATATGGTGACCTGGCTA-CTCGAG-TAGCCAGGTCACCATATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATATGGTGACCTGGCTACTCGAGTAGCCAGGTCACCATATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATATGGTGACCTGGCTACTCGAGTAGCCAGGTCACCATATTTGG-3'

Other clones with same target sequence:

(none)