Construct: shRNA TRCN0000036048

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003034.2-983s1c1
DNA Barcode:
CCCTCCTTTGTCAAGTGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA1 (6489)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Transcript:
RefSeq NM_003034.2 (NON-CURRENT)
Match location:
Position 983 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036048)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCTTTGTCAAGTGAATA
Hairpin Sequence:
5'-CCGG-CCCTCCTTTGTCAAGTGAATA-CTCGAG-TATTCACTTGACAAAGGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCTTTGTCAAGTGAATACTCGAGTATTCACTTGACAAAGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCTTTGTCAAGTGAATACTCGAGTATTCACTTGACAAAGGAGGG-3'

Other clones with same target sequence:

(none)