Construct: shRNA TRCN0000036061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002410.2-3419s1c1
DNA Barcode:
CCTACGAAGAAGCTGATCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGAT5 (4249)
Gene Description:
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
Transcript:
RefSeq NM_002410.2 (NON-CURRENT)
Match location:
Position 3419 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGAAGAAGCTGATCATA
Hairpin Sequence:
5'-CCGG-CCTACGAAGAAGCTGATCATA-CTCGAG-TATGATCAGCTTCTTCGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGAAGAAGCTGATCATACTCGAGTATGATCAGCTTCTTCGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGAAGAAGCTGATCATACTCGAGTATGATCAGCTTCTTCGTAGG-3'

Other clones with same target sequence:

(none)