Construct: shRNA TRCN0000036067

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022120.1-321s1c1
DNA Barcode:
GCTGCTCAGGACCCGCGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OXCT2 (64064)
Gene Description:
3-oxoacid CoA-transferase 2
Transcript:
RefSeq NM_022120.1 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036067)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCAGGACCCGCGTGAA
Hairpin Sequence:
5'-CCGG-GCTGCTCAGGACCCGCGTGAA-CTCGAG-TTCACGCGGGTCCTGAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCAGGACCCGCGTGAACTCGAGTTCACGCGGGTCCTGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCAGGACCCGCGTGAACTCGAGTTCACGCGGGTCCTGAGCAGC-3'

Other clones with same target sequence:

(none)