Construct: shRNA TRCN0000036153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012079.2-562s1c1
DNA Barcode:
GAACCTCATCAAGTATGGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGAT1 (8694)
Gene Description:
diacylglycerol O-acyltransferase 1
Transcript:
RefSeq NM_012079.2 (NON-CURRENT)
Match location:
Position 562 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTCATCAAGTATGGCAT
Hairpin Sequence:
5'-CCGG-GAACCTCATCAAGTATGGCAT-CTCGAG-ATGCCATACTTGATGAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTCATCAAGTATGGCATCTCGAGATGCCATACTTGATGAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTCATCAAGTATGGCATCTCGAGATGCCATACTTGATGAGGTTC-3'

Other clones with same target sequence:

(none)