Construct: shRNA TRCN0000036236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033036.1-606s1c1
DNA Barcode:
CCGCAACTTCTCGGCGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAL3ST3 (89792)
Gene Description:
galactose-3-O-sulfotransferase 3
Transcript:
RefSeq NM_033036.1 (NON-CURRENT)
Match location:
Position 606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAACTTCTCGGCGCACTT
Hairpin Sequence:
5'-CCGG-CCGCAACTTCTCGGCGCACTT-CTCGAG-AAGTGCGCCGAGAAGTTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAACTTCTCGGCGCACTTCTCGAGAAGTGCGCCGAGAAGTTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAACTTCTCGGCGCACTTCTCGAGAAGTGCGCCGAGAAGTTGCGG-3'

Other clones with same target sequence:

(none)