Construct: shRNA TRCN0000036299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_147174.2-1082s1c1
DNA Barcode:
GCCTCTAGTGTAGAGATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HS6ST2 (90161)
Gene Description:
heparan sulfate 6-O-sulfotransferase 2
Transcript:
RefSeq NM_147174.2 (NON-CURRENT)
Match location:
Position 1082 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTAGTGTAGAGATCAAT
Hairpin Sequence:
5'-CCGG-GCCTCTAGTGTAGAGATCAAT-CTCGAG-ATTGATCTCTACACTAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTAGTGTAGAGATCAATCTCGAGATTGATCTCTACACTAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTAGTGTAGAGATCAATCTCGAGATTGATCTCTACACTAGAGGC-3'

Other clones with same target sequence:

(none)