Construct: shRNA TRCN0000036363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153456.2-1011s1c1
DNA Barcode:
CGAGAGTGAAAGAAACACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HS6ST3 (266722)
Gene Description:
heparan sulfate 6-O-sulfotransferase 3
Transcript:
RefSeq NM_153456.2 (NON-CURRENT)
Match location:
Position 1011 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAGTGAAAGAAACACCAT
Hairpin Sequence:
5'-CCGG-CGAGAGTGAAAGAAACACCAT-CTCGAG-ATGGTGTTTCTTTCACTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAGTGAAAGAAACACCATCTCGAGATGGTGTTTCTTTCACTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAGTGAAAGAAACACCATCTCGAGATGGTGTTTCTTTCACTCTCG-3'

Other clones with same target sequence:

(none)