Construct: shRNA TRCN0000036371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007120.2-612s1c1
DNA Barcode:
CCTAAGTTACTAACGACCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A4 (54657)
Gene Description:
UDP glucuronosyltransferase family 1 member A4
Transcript:
RefSeq NM_007120.2 (NON-CURRENT)
Match location:
Position 612 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGTTACTAACGACCAAT
Hairpin Sequence:
5'-CCGG-CCTAAGTTACTAACGACCAAT-CTCGAG-ATTGGTCGTTAGTAACTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGTTACTAACGACCAATCTCGAGATTGGTCGTTAGTAACTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGTTACTAACGACCAATCTCGAGATTGGTCGTTAGTAACTTAGG-3'

Other clones with same target sequence:

(none)