Construct: shRNA TRCN0000036373

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007120.2-259s1c1
DNA Barcode:
TGACAGCCTATGCTGTTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A4 (54657)
Gene Description:
UDP glucuronosyltransferase family 1 member A4
Transcript:
RefSeq NM_007120.2 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036373)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACAGCCTATGCTGTTCCAT
Hairpin Sequence:
5'-CCGG-TGACAGCCTATGCTGTTCCAT-CTCGAG-ATGGAACAGCATAGGCTGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACAGCCTATGCTGTTCCATCTCGAGATGGAACAGCATAGGCTGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACAGCCTATGCTGTTCCATCTCGAGATGGAACAGCATAGGCTGTCA-3'

Other clones with same target sequence:

(none)