Construct: shRNA TRCN0000036395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153612.2-355s1c1
DNA Barcode:
GCCTCTCAAGAAATCCACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HS3ST5 (222537)
Gene Description:
heparan sulfate-glucosamine 3-sulfotransferase 5
Transcript:
RefSeq NM_153612.2 (NON-CURRENT)
Match location:
Position 355 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTCAAGAAATCCACTTT
Hairpin Sequence:
5'-CCGG-GCCTCTCAAGAAATCCACTTT-CTCGAG-AAAGTGGATTTCTTGAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTCAAGAAATCCACTTTCTCGAGAAAGTGGATTTCTTGAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTCAAGAAATCCACTTTCTCGAGAAAGTGGATTTCTTGAGAGGC-3'

Other clones with same target sequence:

(none)