Construct: shRNA TRCN0000036457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002927.3-348s1c1
DNA Barcode:
CTTACTTTGGAGGAAGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS13 (6003)
Gene Description:
regulator of G protein signaling 13
Transcript:
RefSeq NM_002927.3 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACTTTGGAGGAAGTATTA
Hairpin Sequence:
5'-CCGG-CTTACTTTGGAGGAAGTATTA-CTCGAG-TAATACTTCCTCCAAAGTAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACTTTGGAGGAAGTATTACTCGAGTAATACTTCCTCCAAAGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACTTTGGAGGAAGTATTACTCGAGTAATACTTCCTCCAAAGTAAG-3'

Other clones with same target sequence:

(none)