Construct: shRNA TRCN0000036491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005873.1-705s1c1
DNA Barcode:
GAGGCTCATCTACGAGGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS19 (10287)
Gene Description:
regulator of G protein signaling 19
Transcript:
RefSeq NM_005873.1 (NON-CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCTCATCTACGAGGACTA
Hairpin Sequence:
5'-CCGG-GAGGCTCATCTACGAGGACTA-CTCGAG-TAGTCCTCGTAGATGAGCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCTCATCTACGAGGACTACTCGAGTAGTCCTCGTAGATGAGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCTCATCTACGAGGACTACTCGAGTAGTCCTCGTAGATGAGCCTC-3'

Other clones with same target sequence:

  1. TRCN0000300428