Construct: shRNA TRCN0000036506

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005719.2-279s1c1
DNA Barcode:
TGAAGCTGATAGGACCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARPC3 (10094)
Gene Description:
actin related protein 2/3 complex subunit 3
Transcript:
RefSeq NM_005719.2 (NON-CURRENT)
Match location:
Position 279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036506)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGCTGATAGGACCTTGAT
Hairpin Sequence:
5'-CCGG-TGAAGCTGATAGGACCTTGAT-CTCGAG-ATCAAGGTCCTATCAGCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGCTGATAGGACCTTGATCTCGAGATCAAGGTCCTATCAGCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGCTGATAGGACCTTGATCTCGAGATCAAGGTCCTATCAGCTTCA-3'

Other clones with same target sequence:

  1. TRCN0000333049