Construct: shRNA TRCN0000036546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001353.4-625s1c1
DNA Barcode:
GCTGGATTTCTGCAAGTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKR1C1 (1645)
Gene Description:
aldo-keto reductase family 1 member C1
Transcript:
RefSeq NM_001353.4 (NON-CURRENT)
Match location:
Position 625 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATTTCTGCAAGTCAAA
Hairpin Sequence:
5'-CCGG-GCTGGATTTCTGCAAGTCAAA-CTCGAG-TTTGACTTGCAGAAATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATTTCTGCAAGTCAAACTCGAGTTTGACTTGCAGAAATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATTTCTGCAAGTCAAACTCGAGTTTGACTTGCAGAAATCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000333053