Construct: shRNA TRCN0000036660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_373373.1-204s1c1
DNA Barcode:
GAATGGGAAGTTTGTTATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC392549 (392549)
Gene Description:
similar to Glyceraldehyde 3-phosphate dehydrogenase, liver (GAPDH)
Transcript:
RefSeq XM_373373.1 (NON-CURRENT)
Match location:
Position 204 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGGAAGTTTGTTATCAA
Hairpin Sequence:
5'-CCGG-GAATGGGAAGTTTGTTATCAA-CTCGAG-TTGATAACAAACTTCCCATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGGAAGTTTGTTATCAACTCGAGTTGATAACAAACTTCCCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGGAAGTTTGTTATCAACTCGAGTTGATAACAAACTTCCCATTC-3'

Other clones with same target sequence:

(none)