Construct: shRNA TRCN0000036671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002496.1-335s1c1
DNA Barcode:
CATCAACTACCCGTTCGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFS8 (4728)
Gene Description:
NADH:ubiquinone oxidoreductase core subunit S8
Transcript:
RefSeq NM_002496.1 (NON-CURRENT)
Match location:
Position 335 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAACTACCCGTTCGAGAA
Hairpin Sequence:
5'-CCGG-CATCAACTACCCGTTCGAGAA-CTCGAG-TTCTCGAACGGGTAGTTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAACTACCCGTTCGAGAACTCGAGTTCTCGAACGGGTAGTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAACTACCCGTTCGAGAACTCGAGTTCTCGAACGGGTAGTTGATG-3'

Other clones with same target sequence:

  1. TRCN0000307806