Construct: shRNA TRCN0000036677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016246.1-214s1c1
DNA Barcode:
CCGAGTGGTTATCTGCGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSD17B14 (51171)
Gene Description:
hydroxysteroid 17-beta dehydrogenase 14
Transcript:
RefSeq NM_016246.1 (NON-CURRENT)
Match location:
Position 214 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGGTTATCTGCGACAA
Hairpin Sequence:
5'-CCGG-CCGAGTGGTTATCTGCGACAA-CTCGAG-TTGTCGCAGATAACCACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGGTTATCTGCGACAACTCGAGTTGTCGCAGATAACCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGGTTATCTGCGACAACTCGAGTTGTCGCAGATAACCACTCGG-3'

Other clones with same target sequence:

(none)