Construct: shRNA TRCN0000036682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_374855.1-92s1c1
DNA Barcode:
GTGAGTCTGATGGGAGTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC399842 (399842)
Gene Description:
similar to Glutamate dehydrogenase 1, mitochondrial precursor (GDH)
Transcript:
RefSeq XM_374855.1 (NON-CURRENT)
Match location:
Position 92 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGTCTGATGGGAGTATAT
Hairpin Sequence:
5'-CCGG-GTGAGTCTGATGGGAGTATAT-CTCGAG-ATATACTCCCATCAGACTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGTCTGATGGGAGTATATCTCGAGATATACTCCCATCAGACTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGTCTGATGGGAGTATATCTCGAGATATACTCCCATCAGACTCAC-3'

Other clones with same target sequence:

(none)