Construct: shRNA TRCN0000036691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_375958.1-417s1c1
DNA Barcode:
GCTGCTCATCTTCAACACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC400889 (400889)
Gene Description:
similar to proline dehydrogenase (oxidase) 1; proline oxidase 2; p53 induced protein; tumor protein p53 inducible protein 6
Transcript:
RefSeq XM_375958.1 (NON-CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCATCTTCAACACATA
Hairpin Sequence:
5'-CCGG-GCTGCTCATCTTCAACACATA-CTCGAG-TATGTGTTGAAGATGAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCATCTTCAACACATACTCGAGTATGTGTTGAAGATGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCATCTTCAACACATACTCGAGTATGTGTTGAAGATGAGCAGC-3'

Other clones with same target sequence:

(none)