Construct: shRNA TRCN0000036771

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005716.2-1045s1c1
DNA Barcode:
GCCTTTGAAGAGAAGGCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GIPC1 (10755)
Gene Description:
GIPC PDZ domain containing family member 1
Transcript:
RefSeq NM_005716.2 (NON-CURRENT)
Match location:
Position 1045 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036771)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTGAAGAGAAGGCCATT
Hairpin Sequence:
5'-CCGG-GCCTTTGAAGAGAAGGCCATT-CTCGAG-AATGGCCTTCTCTTCAAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTGAAGAGAAGGCCATTCTCGAGAATGGCCTTCTCTTCAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTGAAGAGAAGGCCATTCTCGAGAATGGCCTTCTCTTCAAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000289265