Construct: shRNA TRCN0000036882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004297.2-1247s1c1
DNA Barcode:
GAAGAGAGCAAAGCCTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNA14 (9630)
Gene Description:
G protein subunit alpha 14
Transcript:
RefSeq NM_004297.2 (NON-CURRENT)
Match location:
Position 1247 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000036882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGAGCAAAGCCTTATTT
Hairpin Sequence:
5'-CCGG-GAAGAGAGCAAAGCCTTATTT-CTCGAG-AAATAAGGCTTTGCTCTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGAGCAAAGCCTTATTTCTCGAGAAATAAGGCTTTGCTCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGAGCAAAGCCTTATTTCTCGAGAAATAAGGCTTTGCTCTCTTC-3'

Other clones with same target sequence:

(none)