Construct: shRNA TRCN0000037055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010315.2-255s1c1
DNA Barcode:
GCTGACTTGATGGCCTACTGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gng2 (14702)
Gene Description:
guanine nucleotide binding protein (G protein), gamma 2
Transcript:
RefSeq NM_010315.2 (NON-CURRENT)
Match location:
Position 255 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTTGATGGCCTACTGT
Hairpin Sequence:
5'-CCGG-GCTGACTTGATGGCCTACTGT-CTCGAG-ACAGTAGGCCATCAAGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTTGATGGCCTACTGTCTCGAGACAGTAGGCCATCAAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTTGATGGCCTACTGTCTCGAGACAGTAGGCCATCAAGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000326410