Construct: shRNA TRCN0000037231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153171.2-240s1c1
DNA Barcode:
GCTCCCTTCAAACCTTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rgs13 (246709)
Gene Description:
regulator of G-protein signaling 13
Transcript:
RefSeq NM_153171.2 (NON-CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCCTTCAAACCTTACTTT
Hairpin Sequence:
5'-CCGG-GCTCCCTTCAAACCTTACTTT-CTCGAG-AAAGTAAGGTTTGAAGGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCCTTCAAACCTTACTTTCTCGAGAAAGTAAGGTTTGAAGGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCCTTCAAACCTTACTTTCTCGAGAAAGTAAGGTTTGAAGGGAGC-3'

Other clones with same target sequence:

(none)