Construct: shRNA TRCN0000037289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030564.1-441s1c1
DNA Barcode:
CCCTCAGTTGATGCGACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf34 (80751)
Gene Description:
ring finger protein 34
Transcript:
RefSeq NM_030564.1 (CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGTTGATGCGACTAAA
Hairpin Sequence:
5'-CCGG-CCCTCAGTTGATGCGACTAAA-CTCGAG-TTTAGTCGCATCAACTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGTTGATGCGACTAAACTCGAGTTTAGTCGCATCAACTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGTTGATGCGACTAAACTCGAGTTTAGTCGCATCAACTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000233438