Construct: shRNA TRCN0000037304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030706.1-3074s1c1
DNA Barcode:
CCCATCAGAATCAATGCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim2 (80890)
Gene Description:
tripartite motif-containing 2
Transcript:
RefSeq NM_030706.1 (NON-CURRENT)
Match location:
Position 3074 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCAGAATCAATGCAAAT
Hairpin Sequence:
5'-CCGG-CCCATCAGAATCAATGCAAAT-CTCGAG-ATTTGCATTGATTCTGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCAGAATCAATGCAAATCTCGAGATTTGCATTGATTCTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCAGAATCAATGCAAATCTCGAGATTTGCATTGATTCTGATGGG-3'

Other clones with same target sequence:

  1. TRCN0000308395