Construct: shRNA TRCN0000037316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019912.1-723s1c1
DNA Barcode:
CCCAATCCAGATGATCCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2d2a (56550)
Gene Description:
ubiquitin-conjugating enzyme E2D 2A
Transcript:
RefSeq NM_019912.1 (NON-CURRENT)
Match location:
Position 723 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATCCAGATGATCCTTTA
Hairpin Sequence:
5'-CCGG-CCCAATCCAGATGATCCTTTA-CTCGAG-TAAAGGATCATCTGGATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATCCAGATGATCCTTTACTCGAGTAAAGGATCATCTGGATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATCCAGATGATCCTTTACTCGAGTAAAGGATCATCTGGATTGGG-3'

Other clones with same target sequence:

(none)