Construct: shRNA TRCN0000037348

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178779.2-1052s1c1
DNA Barcode:
GCGTGGTGAAGAGCTCCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf152 (320311)
Gene Description:
ring finger protein 152
Transcript:
RefSeq NM_178779.2 (NON-CURRENT)
Match location:
Position 1052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037348)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGGTGAAGAGCTCCACAT
Hairpin Sequence:
5'-CCGG-GCGTGGTGAAGAGCTCCACAT-CTCGAG-ATGTGGAGCTCTTCACCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGGTGAAGAGCTCCACATCTCGAGATGTGGAGCTCTTCACCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGGTGAAGAGCTCCACATCTCGAGATGTGGAGCTCTTCACCACGC-3'

Other clones with same target sequence:

(none)