Construct: shRNA TRCN0000037350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007579.1-2119s1c1
DNA Barcode:
GCCGTAATGTATCATGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacna1b (12287)
Gene Description:
calcium channel, voltage-dependent, N type, alpha 1B subunit
Transcript:
RefSeq NM_007579.1 (NON-CURRENT)
Match location:
Position 2119 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTAATGTATCATGGGATT
Hairpin Sequence:
5'-CCGG-GCCGTAATGTATCATGGGATT-CTCGAG-AATCCCATGATACATTACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTAATGTATCATGGGATTCTCGAGAATCCCATGATACATTACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTAATGTATCATGGGATTCTCGAGAATCCCATGATACATTACGGC-3'

Other clones with same target sequence:

(none)