Construct: shRNA TRCN0000037412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014006.2-6826s1c1
DNA Barcode:
CGGCAAATCATATTTCCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMG1 (23049)
Gene Description:
SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Transcript:
RefSeq NM_014006.2 (NON-CURRENT)
Match location:
Position 6826 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAATCATATTTCCAGAA
Hairpin Sequence:
5'-CCGG-CGGCAAATCATATTTCCAGAA-CTCGAG-TTCTGGAAATATGATTTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAATCATATTTCCAGAACTCGAGTTCTGGAAATATGATTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAATCATATTTCCAGAACTCGAGTTCTGGAAATATGATTTGCCG-3'

Other clones with same target sequence:

(none)