Construct: shRNA TRCN0000037434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376950.1-872s1c1
DNA Barcode:
CCTGAAGACAATGGAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC401568 (401568)
Gene Description:
similar to MGC43306 protein
Transcript:
RefSeq XM_376950.1 (NON-CURRENT)
Match location:
Position 872 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGACAATGGAGGAGAA
Hairpin Sequence:
5'-CCGG-CCTGAAGACAATGGAGGAGAA-CTCGAG-TTCTCCTCCATTGTCTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGACAATGGAGGAGAACTCGAGTTCTCCTCCATTGTCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGACAATGGAGGAGAACTCGAGTTCTCCTCCATTGTCTTCAGG-3'

Other clones with same target sequence:

(none)