Construct: shRNA TRCN0000037444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_373109.2-1221s1c1
DNA Barcode:
CCTATATGCTACTTAGCGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYLK4 (340156)
Gene Description:
myosin light chain kinase family member 4
Transcript:
RefSeq XM_373109.2 (NON-CURRENT)
Match location:
Position 1221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATATGCTACTTAGCGGTT
Hairpin Sequence:
5'-CCGG-CCTATATGCTACTTAGCGGTT-CTCGAG-AACCGCTAAGTAGCATATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATATGCTACTTAGCGGTTCTCGAGAACCGCTAAGTAGCATATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATATGCTACTTAGCGGTTCTCGAGAACCGCTAAGTAGCATATAGG-3'

Other clones with same target sequence:

(none)