Construct: shRNA TRCN0000037527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020791.1-2327s1c1
DNA Barcode:
GCCTAAGAGTTTGAAGTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK1 (57551)
Gene Description:
TAO kinase 1
Transcript:
RefSeq NM_020791.1 (NON-CURRENT)
Match location:
Position 2327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAAGAGTTTGAAGTCTAA
Hairpin Sequence:
5'-CCGG-GCCTAAGAGTTTGAAGTCTAA-CTCGAG-TTAGACTTCAAACTCTTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAAGAGTTTGAAGTCTAACTCGAGTTAGACTTCAAACTCTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAAGAGTTTGAAGTCTAACTCGAGTTAGACTTCAAACTCTTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000363677