Construct: shRNA TRCN0000037593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002625.1-659s1c1
DNA Barcode:
CTAAAGAGAATTGAGTGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKFB1 (5207)
Gene Description:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Transcript:
RefSeq NM_002625.1 (NON-CURRENT)
Match location:
Position 659 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAAGAGAATTGAGTGCTAT
Hairpin Sequence:
5'-CCGG-CTAAAGAGAATTGAGTGCTAT-CTCGAG-ATAGCACTCAATTCTCTTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAAGAGAATTGAGTGCTATCTCGAGATAGCACTCAATTCTCTTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAAGAGAATTGAGTGCTATCTCGAGATAGCACTCAATTCTCTTTAG-3'

Other clones with same target sequence:

(none)