Construct: shRNA TRCN0000037630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020240.1-91s1c1
DNA Barcode:
GAGCCCACAAACTTTGTGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42SE2 (56990)
Gene Description:
CDC42 small effector 2
Transcript:
RefSeq NM_020240.1 (NON-CURRENT)
Match location:
Position 91 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCCCACAAACTTTGTGCAT
Hairpin Sequence:
5'-CCGG-GAGCCCACAAACTTTGTGCAT-CTCGAG-ATGCACAAAGTTTGTGGGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCCCACAAACTTTGTGCATCTCGAGATGCACAAAGTTTGTGGGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCCCACAAACTTTGTGCATCTCGAGATGCACAAAGTTTGTGGGCTC-3'

Other clones with same target sequence:

(none)