Construct: shRNA TRCN0000037634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000167.3-814s1c1
DNA Barcode:
CCGGAGTTCTTCTGAGATCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK (2710)
Gene Description:
glycerol kinase
Transcript:
RefSeq NM_000167.3 (NON-CURRENT)
Match location:
Position 814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGTTCTTCTGAGATCTA
Hairpin Sequence:
5'-CCGG-CCGGAGTTCTTCTGAGATCTA-CTCGAG-TAGATCTCAGAAGAACTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGTTCTTCTGAGATCTACTCGAGTAGATCTCAGAAGAACTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGTTCTTCTGAGATCTACTCGAGTAGATCTCAGAAGAACTCCGG-3'

Other clones with same target sequence:

(none)