Construct: shRNA TRCN0000037650

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021733.1-1262s1c1
DNA Barcode:
GAAACCCATTCTGGAGGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSKS (60385)
Gene Description:
testis specific serine kinase substrate
Transcript:
RefSeq NM_021733.1 (NON-CURRENT)
Match location:
Position 1262 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037650)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACCCATTCTGGAGGAGTT
Hairpin Sequence:
5'-CCGG-GAAACCCATTCTGGAGGAGTT-CTCGAG-AACTCCTCCAGAATGGGTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACCCATTCTGGAGGAGTTCTCGAGAACTCCTCCAGAATGGGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACCCATTCTGGAGGAGTTCTCGAGAACTCCTCCAGAATGGGTTTC-3'

Other clones with same target sequence:

(none)