Construct: shRNA TRCN0000037672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000189.4-3937s1c1
DNA Barcode:
CACTGTGAAGTTGGCCTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HK2 (3099)
Gene Description:
hexokinase 2
Transcript:
RefSeq NM_000189.4 (NON-CURRENT)
Match location:
Position 3937 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGTGAAGTTGGCCTCATT
Hairpin Sequence:
5'-CCGG-CACTGTGAAGTTGGCCTCATT-CTCGAG-AATGAGGCCAACTTCACAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGTGAAGTTGGCCTCATTCTCGAGAATGAGGCCAACTTCACAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGTGAAGTTGGCCTCATTCTCGAGAATGAGGCCAACTTCACAGTG-3'

Other clones with same target sequence:

(none)